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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs

Fig. 1

An automated platform for high-throughput in situ profiling of chromatin proteins. a AutoCUT&RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT&RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. b Hierarchically clustered correlation matrix of AutoCUT&RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log2-transformed values of read counts split into 500 bp bins across the genome

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