Fig. 3

The expression of ex14D-Ezh2 is activated by MYBL1. a The epigenetic signature and transcription factor binding motifs around Ezh2’s 5′ UTR. HOMER-motif analysis detected E2F1 binding sites within the 300 bp upstream of the ex1a TSS. ex1a is the first exon that is known for coding ex14-Ezh2’s 5′ UTR. ex1b is a putative exon for ex14D-Ezh2’s 5′ UTR. b Characterization of ex14D-Ezh2’s 5′ UTR. Primer p1 and p2 were used to amplify the DNA sequence coding the transcripts initiated from the ex1b 5′ UTR. The amplicons were cloned into vectors and transformed into bacteria. The alternative splicing events were assessed by genotyping single colonies. c Cryosections of p30 testes were immunostained with indicated proteins. Scale bar: 20 μm