Fig. 2
The expression of ex14D-Ezh2 in spermatocytes is responsible for the establishment of H3K27me2. a–c The dynamics of H3K27me3 and H3K4me3 enrichment on E2f1-3 during spermatogenesis. Spermatocytes were isolated from postnatal day 12 (p12), 17 (p17), and 30 (p30) testes for ChIP-seq analysis. d–f RNA-seq analysis of E2f1-3 transcription in germ cells purified from p9 and p17 testes. The representative sample tracks for each time point are presented here. g Assessment of H3K27 methylation in different meiotic cell populations. Cryosections of 4-month-old testis were immunostained for indicated proteins. (l) leptotene spermatocytes; (z) zygonema; (p) pachynema; (d) diplonema. Scale bar: 20 μm