Table 1 Primer sequences for RT-PCR analysis

odc

5′-CAG CTA GCT GTG GTG TGG-3′

5′-CAA CAT GGA AAC TCA CAC C-3′

sox3

5′-AGC GCT TTC TCG TGC AGT-3′

5′-TGC CAG CAG GCA AGT AAA-3′

pax3

5′-AGG AGG ACA TGG AGC TGG AT-3′

5′-AAC GGG TAA AGG TTC GCT GT-3′

twist

5′-CAG GAA GAG TCC AGC TCG C-3′

5′-GTG GCC TGA GCT GTA GTG G-3′

slug

5′-CCC CCT CCA CAA TCT GAC AC-3′

5′-GCC ACG GTC TAG AAA AGG CT-3′

sox8

5′-TGT CTC CAG CCG GAT CAG A-3′

5′-CAG CCT CCT CCA CAA AGG G-3′

sox9

5′-TTG GTG AGC TGA GCA GTG AG-3′

5′-GCT GTT GCT GTT GGT CAC TG-3′

sox10

5′-TAT GGT ATG GGC CCA GGC T-3′

5′-GGG TAG GGG GTC CAT GAC T-3′

otx2

5′-TTC CTT CGC GGA TTG TGC T-3′

5′-GAC CAG GGT TCT GTG TGG G-3′

pax6

5′-CAG AAC AGT CAC AGC GGA GT-3′

5′-TCACTG CCG GGT ACT TGA AC-3′

rax

5′-TCC CCT GAT GGC TGA TGG A-3′

5′-GGC ATG GTG GCT GAT CTG T-3′

epha4

5′-CCC AGC AGA ATG GCC TGA A-3′

5′-GGC TGG CTC CTT CAC CAA T-3′

epha2

5′-GTA CCC ATT GGC CAC TGC T-3′

5′-TGA TGG TGA TGG TGG TGC C-3′

ephB2

5′-AGG ACT GCG ATC TCC TGG T-3′

5′-TTT GCT GGG CTC TGA GGT G-3′

aldh1a2

5′-GAC TGC TCT TGC GAC CCT T-3′

5′-GCT CCT GCA GTT GGA CCA T-3′

wnt8a

5′-GCG GCT GCA GTG ATA ATG C-3′

5′-ACT CTC GTC CCT CTG TCC C-3′