Fig. 7

KDM5C is involved in eye field induction and differentiation. a kdm5c MO (13 ng) was coinjected with β-galactosidase mRNA into one blastomere of eight-cell stage embryos. Embryos were fixed at the neurula stage (st. 16). β-galactosidase staining indicates the injected side of the embryos. WISH analysis was performed using otx2, rax, and pax6 markers. otx2, rax, and pax6 expressions were downregulated on the injected side of the embryos. Changes in expression levels of neural and eye-specific markers were efficiently rescued by coinjecting kdm5c MO and kdm5c* RNA. b Statistical analysis of embryos exhibiting abnormal expression patterns of eye field induction and differentiation markers compared with that of control embryos. c WISH analysis of otx2, rax, and pax6 at stage 32 of developing embryos is in agreement with the analysis performed at stage 16 of developing embryos. Downregulated expression on the injected side of the embryos indicated that kdm5c is significant for eye field induction and differentiation. d Statistical analysis of embryos exhibiting abnormal expression patterns of eye field induction and differentiation markers compared with that of control embryos. e WISH analysis of stage 32 embryos using the lens-specific marker cryba1 indicated that expression of cryba1 is not affected by kdm5c depletion. f Percentage of embryos with reduced expression indicated that cryba1 expression was not affected by kdm5c knockdown. g RT-PCR analysis using eye field induction and differentiation markers as well as cryba1 revealed that KDM5C downregulated the expression of otx2, rax, and pax6 but not cryba1, which remained unaffected. ns, not significant; **P < 0.01; CTL, control