Fig. 3

TUNEL and pH3 staining of kdm5c-depleted embryos at late tailbud stage (st. 32) of embryonic development. a kdm5c MO leads to an increase in TUNEL-positive cells on the injected side of embryos compared with the uninjected side. No increase is observed in control MO-injected embryos. b Statistical analysis of kdm5c-depleted embryos and control embryos showing a significant increase in TUNEL-positive cells in the kdm5c MO-injected side compared with uninjected side. c Depletion of kdm5c results in significant reduction in cell proliferation indicated by pH3-positive cells in the injected side of the embryos compared with the uninjected side. d Statistical quantification revealing marked reduction in pH3-positive cells in the kdm5c MO-injected side compared with the uninjected side of the embryos. No significant decrease was observed between control embryos. ns, not significant; ****P < 0.0001. CTL, control