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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Physiological effects of KDM5C on neural crest migration and eye formation during vertebrate development

Fig. 2

Knockdown of kdm5c induced phenotypic malformations. a kdm5c morpholino oligonucleotide (MO; 48 ng) was injected into embryos at the one-cell stage, and then, embryos were fixed at stage 46. kdm5c-depleted embryos exhibited a reduction in head size area (indicated by the dotted red circle) compared with that of control embryos. Small-sized heads of kdm5-morphant embryos were effectively rescued by injecting mutant kdm5c (kdm5c*) along with kdm5c MO. Scale bar = 500 µm. b Graphical representation of embryos with reduced head formation compared with control. More than 80% of kdm5c MO-injected embryos exhibited small-sized heads. Small-sized heads were effectively rescued by coinjection with kdm5c* RNA. c A graph showing the reduction in head area in kdm5c morphants. Compared with the control embryos, kdm5c-morphant embryos showed approximately 70% reduction in head area, and this phenotypic abnormality was rescued in approximately 88% of embryos coinjected with kdm5c MO and kdm5c* RNA. d Analysis of cartilage formation in kdm5c morphants was performed by fixing the embryos at stage 46 and then staining with alcian blue. Alcian blue staining of kdm5c MO-injected embryos demonstrated reduced cartilage size compared with that of control embryos. This phenotypic anomaly was efficiently recovered by rescue experiments. Scale bar = 500 µm. **P < 0.01, ****P < 0.0001; nonparametric, one-tailed Mann–Whitney rank-sum test; ba, branchial arches; CTL, control; ta, tectum anterious; mc, Meckel’s cartilage; ic, infrarostral cartilage

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