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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: ZEB2 stably represses RAB25 expression through epigenetic regulation by SIRT1 and DNMTs during epithelial-to-mesenchymal transition

Fig. 1

ZEB2 and RAB25 expression are inversely correlated. (a, b) Relative mRNA expression of ZEB2, RAB25 and CDH1 measured by qRT-PCR in (a) MCF7, HT29 and A431 ZEB2 doxycycline-inducible (+dox) models and b MDA-MB-231 ZEB2 KD (shZEB2). Control values were set to 1, and s.d. is shown. P values were determined using unpaired t tests (*p < 0.05;**p < 0.01). Three independent experiments were performed. c ZEB2 and RAB25 mRNA expression in a epithelial cell line panel. de Correlation between ZEB2 (y-axis) and RAB25 (x-axis) expression from publicly available datasets from d NCI-60 cell panel and e the Cancer Cell Line Encyclopedia (CCLE). Mean of each parameter was calculated, individual cell type values reported to the mean and log2 transformed. Pearson’s correlation test was used to calculate r and p values. f Boxplots showing RAB25 (left panel) and ZEB2 (right panel) expression in epithelial (n = 11) and mesenchymal (n = 37) cell lines from NCI-60 cell panel. P values were determined using unpaired t tests (***p < 0.001)

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