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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: High-resolution mapping of centromeric protein association using APEX-chromatin fibers

Fig. 6

Endogenous CENP-B and CENP-I extend further than the CENP-A domain on TEEN fibers. Representative images of chromatin fibers prepared from U2OS cells using the TEEN buffer and stained for CENP-A and either CENP-B (a) or CENP-I (b). Scale bar: 2.5 μm. Intensity plots for CENP-A and CENP-B or CENP-I gray values along the length of the fiber (in μm) are shown on the right. c Scatter plot depicting the distance of spreading from the edges of the CENP-A domain for CENP-C, CENP-H, CENP-T, CENP-B and CENP-I. Each fiber is represented by two dots, for the distance of spreading left and right of the centromere domain. Error bars represent mean with SD. FDR adjusted Kolmogorov–Smirnov p values < 0.001 are represented as **. Not significant p values > 0.05 are not depicted. n = 142 fibers for CENP-B (from three experiments), 160 fibers for CENP-C (from two experiments), 189 fibers for CENP-H (from two experiments), 177 fibers for CENP-I (from two experiments) and 142 fibers for CENP-T (from two experiments)

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