Fig. 1
From: High-resolution mapping of centromeric protein association using APEX-chromatin fibers
Comparison of different methods for chromatin fiber preparation. a Scatter plot depicting the size of CENP-A domain on chromatin fibers prepared with three different methods (left panel). Each dot represents one fiber. Error bars represent mean with SD. n = 32, 28 and 37 fibers for TEEN buffer, single lysis step and double lysis , respectively, from one experiment. Representative pictures of fibers (right panel). Scale bar: 2.5 μm. b Representative pictures of chromatin fibers prepared with three different methods and stained for CENP-A and either CENP-B or CENP-C. Scale bar: 2.5 μm