Fig. 6

Methylation of ATF7IP plays a role in the silencing transgene integrated by retrovirus vector in mESCs. a MSCV-GFP expression was analyzed by flow cytometric analysis at indicated time points. The transfected cells were subjected to continuous drug selection from day 2 after the transfection. b RT-qPCR analysis was performed with samples collected at day 4 after the transfection. RNA expression was normalized to Hprt expression and is shown relative to the level in WT cells without the case of 3xFLAG-ATF7IP. For 3xFLAG-ATF7IP mRNA, RNA expression is shown relative to the level in the ATF7IP WT-rescued cells. Data are mean ± SEM; n = 4, biological replicates. NS: P > 0.05, *P < 0.05 by unpaired Student’s t test