Fig. 4
From: Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8
Interaction between the G9a SET-domain and peptides for histone H3, ATF7IP, or LIG1. a An alignment of the sequences for histone H3, mouse/human ATF7IP, mouse/human LIG1, and ATF7IP mutants. Underline indicates the sequence for the peptide used in this study. The methylated lysine was indicated in green. b SPR measurements of the interaction with the G9a SET-domain and peptides. Data are mean ± SE. KD values were calculated from the kon and koff values when they are indicated. When kinetics were not fitted, kon and koff are shown in (–) and KD values were calculated by steady-state affinity analysis. c 3xFLAG-tagged ATF7IP was immunopurified from mESC lines and subjected to western blot analysis. LIG1 (: 3xF-ATF7IP) indicates a chimeric protein composed of mLIG1 (residues 127–154) and ATF7IP (residues 29–1306). d 3xFLAG-tagged ATF7IP was immunopurified from mESC lines and subjected to western blot analysis. 8–1306 (: 3xF-ATF7IP) indicates a deletion mutant of ATF7IP (residues 8–1306)