Fig. 5
From: Oxidative stress in sperm affects the epigenetic reprogramming in early embryonic development
Replacement of cytosines in pre-replicative zygotes. a Schema describes the strategy to measure replication and DNA demethylation via the incorporation of EdU and EdC in zygotes and its readout. Representative images showing zygotes with EdU (green) and EdC (green) incorporation 12 h after IVF. Numbers refer to zygotes showing EdU or EdC incorporation relative to the total number of analysed zygotes, respectively. b Schema describes the strategy to measure replication and DNA demethylation via the incorporation of BrdU and EdC in zygotes and its readout. Representative images showing zygotes stained for BrdU (red) and EdC (green) incorporation 12 h after IVF. Numbers refer to zygotes positive for EdC signal and negative for BrdU signal relative to the total number of analysed zygotes. Zygotes 24 h pIVF were stained and used as post-replicative control zygotes. Scale bars, 50 μm