Fig. 5

Analysis of a global transcriptional and b DNA methylation changes on X-chromosome and autosomes in animals lacking Xist. a Left: Scatter plot of RNA expression levels for genes autosomes (black) and X-chromosome (red) (N = 3 for both mutant and control animals). The genes on X-chromosome exhibit an upward shift relative to autosomes. Middle: Cumulative expression plots of fold expression changes (mutant/control) for genes on autosomes (black) and X-chromosome (red). The genes on X-chromosome exhibit a rightward shift (p < 0.01, Wilcoxon rank-sum test). Right: Binned fold changes in expression for genes on autosomes (black) and X-chromosome (red). b Left: Scatter plot of DNA methylation abundance at CpG islands on autosomes (black) and X-chromosome (red) (N = 2 for both mutant and control animals). The CpG islands on X-chromosome exhibit a downward shift relative to CpG islands on autosomes. Middle: Cumulative plots of fold methylation abundance changes (mutant/control) for CpG islands on autosomes (black) and X-chromosome (red) (p < 0.01, Wilcoxon rank-sum test). Right: Binned fold changes in methylation abundance at CpG islands on autosomes (black) and X-chromosome (red)