Fig. 4

a GFP IHC in different brain regions including cortex, hypocampus, brainstem and cerebellum (clockwise starting from left upper panel) of Nestin-Cre+ Xist^mut/MeCP2-GFP Xist^2lox animals lacking Xist in the brain. Bar indicates 80 µm. b Percentage of GFP-positive cells in the indicated brain regions in all cells, NeuN-positive neurons and S100b-positive astrocytes (n = 4, error bar indicates SD, neurons vs astrocytes, *p < 0.05, Student’s t test). c Reactivation of MeCP2-GFP reporter gene in neurons and astrocytes in brains from Nestin-Cre+ Xist^mut/MeCP2-GFP Xist^2lox animals. Double IF for GFP and NeuN (upper panel) and for GFP and S100b (lower panel) demonstrates GFP IF in both NeuN-positive neurons and S100b-positive astrocytes