Fig. 2
SETDB1 loss is coupled with expansion of the Xi territory. a Distribution of heterochromatin bands on the Xi in parental RPE1 cells and SETDB1-targeted clone S40. The top panels show indirect immunofluorescence patterns of H3K27me3 (Green) merged with DAPI (blue), whereas lower panels show representative examples of indirect immunofluorescence pattern of H3K27me3 (green) merged with the pattern of H3K9me3 detected by direct immunofluorescence (red). b Indirect immunofluorescence showing DAPI (blue), the distribution of HP1β (Red), H3K27me3 (green), and a merge of the two in RPE1 and two SETDB1 KO clones. White arrow heads indicate the location of the Xi. c Volume measurements of the Xi in RPE1 compared to the three KO clones displayed as oneway ANOVA. P-values calculated using Tukey–Kramer honest significant difference. Xi/DAPI volumes are indicated on the y-axis. Each black dot indicates a measurement made for individual nuclei in each sample. The green diamonds show the mean (central horizontal line) and 95% confidence interval between the apexes of the diamond. The width of the diamond is proportional to the sample size with wider diamonds indicating more measured nuclei. Circles on the far right show all pairs Tukey–Kramer 0.05 p value spread with significance presented by the angle of intersection between circles