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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Loss of SETDB1 decompacts the inactive X chromosome in part through reactivation of an enhancer in the IL1RAPL1 gene

Fig. 1

Promoter-trap targeting of exon-2 results in loss of SETDB1 protein. a Schematic map of the SETDB1 locus corresponding to Chr1: 150,926,412–150,964,744 bp, hg38. Vertical lines correspond to the 22 exons of SETDB1. Exon-2 that was the target for TALEN cutting is indicated as the location of primers used for RT-PCR between exons 1–3 and 18–21. b Ethidium bromide-stained agarose gel image of RT-PCR data for the samples indicated. Annotation of +RT indicates cDNA prepared with reverse transcriptase, whereas − RT was without, serving as a control for genomic DNA carryover. Size in base pairs is indicated to the right. c qRT-PCR data for SETDB1 shown relative to GAPDH expression (y-axis). Bars correspond to combined data from three biological replicates, each performed as three technical replicates. Error bars show standard error of the mean, and p values were derived from performing unpaired t tests. d SETDB1 locus RNA-Seq data for RPE1 and SETDB1 KO clones showing forward (+)- and reverse (−)-strand profiles for the same interval as above in part-a. e Western blot showing absence of SETDB1 protein in three independent knockout clones. Molecular weight in kD is indicated on the left. The loading levels are indicated by the β-actin signal

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