Table 1 Antibodies used in this study
Antibody | Brand | Cat no. | Use | Condition |
|---|---|---|---|---|
EZH2 | BD | 612666 | WB, IF | 1:2000, 1:200 |
EZH2 (AC22) | Merck Millipore | 17-662 | IP | 2/500 μg |
EZH2 | R&D | AF4767 | PLA | 1:50 |
Histone H3 | Abcam | ab24834 | WB | 1:1000 |
α-Tubulin | Abcam | ab52866 | WB, IF | 1:5000, 1:500 |
H3K27me3(C36B11) | Cell signalling | 9733 | WB | 1:2000 |
PCNA (PC10) | Cell signalling | 2586 | WB, IF, PLA, IP | 1:5000, 1:200 |
Pan-methyl lysine | Abcam | ab7315 | WB | 1:2000 |
DNA Polymerase delta (607) | Abcam | ab10362 | WB | 1:1000 |
CYCLIN A2 | Abcam | ab38 | WB | 1:500 |
CYCLIN D1 | Abcam | ab40754 | WB | 1:5000 |
FLAG-tag | Sigma | F7425 | WB, IP | 1:2000, 1:100 |
Myc-Tag | Cell signalling | 2278 | WB, IP | 1:4000, 1:200 |
Ubiquityl-PCNA (Lys164) (D5C7P) | Cell signalling | 13439 | WB | 1:1000 |
Histone H2AX | Abcam | Ab11175 | WB | 1:2500 |
γ-H2A.X | Cell signalling | 9718 | WB | 1:2000 |
Goat anti-rabbit IgG (HRP) | Abcam | ab6721 | WB | 1:10,000 |
Goat anti-mouse IgG (HRP) | Abcam | ab6789 | WB | 1:10,000 |
Rabbit anti-goat IgG-FITC | Boster | BA1110 | IF | 1:60 |
Rabbit anti-goat IgG-Cy3 | Boster | BA1034 | IF | 1:60 |
Goat anti-mouse IgG-Cy3 | Boster | BA1031 | IF | 1:60 |
Goat anti-mouse IgG-FITC | Boster | BA1101 | IF | 1:60 |
Goat anti-rabbit IgG-Cy3 | Boster | BA1032 | IF | 1:60 |
Donkey anti-mouse IgG (H + L)(Alexa Fluor 555) | Beyotime | A0460 | IF | 1:500 |
Anti-IdU | Novus | NBP2-44056 | DNA fibre | 1:300 |