Skip to main content

Advertisement

Table 1 Antibodies used in this study

From: EZH2 promotes DNA replication by stabilizing interaction of POLδ and PCNA via methylation-mediated PCNA trimerization

Antibody Brand Cat no. Use Condition
EZH2 BD 612666 WB, IF 1:2000, 1:200
EZH2 (AC22) Merck Millipore 17-662 IP 2/500 μg
EZH2 R&D AF4767 PLA 1:50
Histone H3 Abcam ab24834 WB 1:1000
α-Tubulin Abcam ab52866 WB, IF 1:5000, 1:500
H3K27me3(C36B11) Cell signalling 9733 WB 1:2000
PCNA (PC10) Cell signalling 2586 WB, IF, PLA, IP 1:5000, 1:200
Pan-methyl lysine Abcam ab7315 WB 1:2000
DNA Polymerase delta (607) Abcam ab10362 WB 1:1000
CYCLIN A2 Abcam ab38 WB 1:500
CYCLIN D1 Abcam ab40754 WB 1:5000
FLAG-tag Sigma F7425 WB, IP 1:2000, 1:100
Myc-Tag Cell signalling 2278 WB, IP 1:4000, 1:200
Ubiquityl-PCNA (Lys164) (D5C7P) Cell signalling 13439 WB 1:1000
Histone H2AX Abcam Ab11175 WB 1:2500
γ-H2A.X Cell signalling 9718 WB 1:2000
Goat anti-rabbit IgG (HRP) Abcam ab6721 WB 1:10,000
Goat anti-mouse IgG (HRP) Abcam ab6789 WB 1:10,000
Rabbit anti-goat IgG-FITC Boster BA1110 IF 1:60
Rabbit anti-goat IgG-Cy3 Boster BA1034 IF 1:60
Goat anti-mouse IgG-Cy3 Boster BA1031 IF 1:60
Goat anti-mouse IgG-FITC Boster BA1101 IF 1:60
Goat anti-rabbit IgG-Cy3 Boster BA1032 IF 1:60
Donkey anti-mouse IgG (H + L)(Alexa Fluor 555) Beyotime A0460 IF 1:500
Anti-IdU Novus NBP2-44056 DNA fibre 1:300