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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: EZH2 promotes DNA replication by stabilizing interaction of POLδ and PCNA via methylation-mediated PCNA trimerization

Fig. 1

EZH2-knockdown hDPCs show decreased proliferation, but no transcriptionally significant changes are observed in DNA replication-related genes. a Quantitative PCR and western blot show EZH2 knockdown with shRNA in hDPCs (72 h after transduction). b Colony formation assay by crystal violet staining demonstrates the viability of EZH2 knockdown hDPCs. c Growth curves measured with CCK-8. d Flow cytometry analysis shows the cell cycle distribution of EZH2-knockdown hDPCs. e Cell cycle RNA-sequencing assay shows the transcripts with significantly altered expression in EZH2-knockdown hDPCs. f Quantitative PCR verifies the expression levels of genes identified in the RNA-Seq data. (ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001)

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