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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: DNA methylation analysis on purified neurons and glia dissects age and Alzheimer’s disease-specific changes in the human cortex

Fig. 1

Experimental setup and cell-type-specific DNA methylation signatures. a General work flow scheme for neuron and glia cell-type separation and DNA methylation profiling. b Cluster analysis on complete data shows full separation of neuron and glia samples. c Volcano plot for identification of 57,908 cell-type-specific CpGs (ct-DMCGs, dark red) in sorted CTRLs. d Genomic distribution of ct-DMCGs that are hypo- or hypermethylated in neurons. CpGs are classified in respect of hypo-or hypermethylation in neurons compared to glia. e Relative distribution of ct-DMCGs in relation to array design. f NGS-based validation for exemplary ct-DMCGs in a subset of neuron and glia samples. Columns represent methylation levels of cytosines at CpGs (upper panels) or CpAs (lower panels). Rows are samples ordered by similarity. Black triangles mark CpGs that are present on the 450k array. NC/GC: neuron/glia controls, N3-N6/G3-G6: neuron/glia Braak stages III–VI. g Similarity of neuronal methylation profiles (CTRLs only) for our own study (dataset 1) and data from Guintivano et al [35] (dataset 2). h Overlap of ct-DMCGs as defined by datasets 1 and 2, respectively

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