Fig. 5
Comparison of subtelomeric chromatin structure in somatic and ovarian tissues. a HP1, H3K9me3, and Rhi occupancy at the EY03383 transgene located in the 2R TAS was estimated by ChIP-qPCR. rp49 and metRS-m regions are used as negative controls. b DNA FISH with TAS 2R-3R probe (green) combined with Rhi (red) or H3K27me3 (red) staining was done on ovaries of yw and spn-E1/spn-Ehls3987 strains. Nuclei of nurse cells (stage VIII–X) are shown. c RT-qPCR analysis of the expression levels of transgenic mini-white in ovaries of transgenic strains. white-specific primers detect only transgenic transcripts because endogenous white is partially deleted