Fig. 3From: Key role of piRNAs in telomeric chromatin maintenance and telomere nuclear positioning in Drosophila germlineRole of piRNA pathway in the deposition of HP1, Rhi, and H3K9me3 at telomeric transgenes and endogenous telomeric retrotransposons in ovaries. a ChIP-qPCR analysis of HP1, Rhi, and H3K9me3 enrichment at EY08176 transgene (insTAHRE), endogenous HeT-A, TART-A, TAHRE and a set of dual-strand piRNA clusters in ovaries of hetero- and trans-heterozygous (spn-E1/spn-Ehls3987) spindle-E mutants. Asterisks indicate statistically significant differences in chromatin protein levels at the indicated regions between spnE/+ and spnE/spnE (*P < 0.05 to 0.01, **P < 0.01 to 0.001, ***P < 0.001, unpaired t test). b ChIP-qPCR analysis of HP1, Rhi, and H3K9me3 enrichment at EY00453 (ins2TART-B), endogenous HeT-A, TART-B, and dual-strand piRNA cluster 6 in ovaries upon spnE germline knockdown. Here, we used primers specific to the transgene insertion site instead of those to 5′P due to the presence of additional P-element-based constructs in the genome. The TART-B promoter was amplified using a primer pair surrounding the insTART-B insertionBack to article page