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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Key role of piRNAs in telomeric chromatin maintenance and telomere nuclear positioning in Drosophila germline

Fig. 3

Role of piRNA pathway in the deposition of HP1, Rhi, and H3K9me3 at telomeric transgenes and endogenous telomeric retrotransposons in ovaries. a ChIP-qPCR analysis of HP1, Rhi, and H3K9me3 enrichment at EY08176 transgene (insTAHRE), endogenous HeT-A, TART-A, TAHRE and a set of dual-strand piRNA clusters in ovaries of hetero- and trans-heterozygous (spn-E1/spn-Ehls3987) spindle-E mutants. Asterisks indicate statistically significant differences in chromatin protein levels at the indicated regions between spnE/+ and spnE/spnE (*P < 0.05 to 0.01, **P < 0.01 to 0.001, ***P < 0.001, unpaired t test). b ChIP-qPCR analysis of HP1, Rhi, and H3K9me3 enrichment at EY00453 (ins2TART-B), endogenous HeT-A, TART-B, and dual-strand piRNA cluster 6 in ovaries upon spnE germline knockdown. Here, we used primers specific to the transgene insertion site instead of those to 5′P due to the presence of additional P-element-based constructs in the genome. The TART-B promoter was amplified using a primer pair surrounding the insTART-B insertion

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