Fig. 5

ChIP analysis of c-Myb occupancy and H3K27ac levels at the mim-1 locus. a Map of the mim-1 locus (LECT2 leucocyte cell derived chemotaxin 2 (Gallus gallus (Chicken)). The enhancer mapped in [28] is indicated. Myb recognition elements (MREs) are indicated by colour-coded asterisk (red: the strong MRE = YAACGG [27], green: MRE = YAACNG and blue: double MREs with palindromic orientation). The amplicons used for qRT-PCR quantification are indicated. b, c Occupancy of c-Myb wild-type (hcM) and D152V as measured by ChIP and qRT-PCR at the indicated regions. IgG IP controls are included. Bar graphs represent the mean ± SEM of three independent biological replicates each measured in triplicate. d Fragmentation of chromosomal DNA of one replicate. e–g Levels of H3K27ac as measured by ChIP and qRT-PCR at the indicated regions. Bar graphs represent the mean ± SEM of two independent biological replicates each measured in triplicate. ChIP was performed as described on HD11 cells 24 h after transfection