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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: The pioneer factor activity of c-Myb involves recruitment of p300 and induction of histone acetylation followed by acetylation-induced chromatin dissociation

Fig. 2

c-Myb histone binding is impaired by specific histone modifications. a Schematic figure of the full-length c-Myb protein and the regions fused to GST in GST pull-down assays and peptide binding assays. The D152V mutation is indicated with an arrow. b Peptide arrays containing 384 different histone tail modification combinations incubated with GST, GST-c-Myb-NR123 or GST-c-Myb-NR123-D152V and detected with anti-GST primary antibody (A00865, GenScript). The locations of H3R26 citrullination, acetylation and H3S28 phosphorylation (alone and in combinations with other modifications) are indicated with yellow, red and blue circles, respectively. An enlarged version of the region containing these modifications for the array incubated with GST-c-Myb-NR123 without annotations is also shown

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