Fig. 1

Histone acetylation prevents c-Myb binding to native K562 histones. a 18% SDS-PAGE of native histones isolated from K562 cells treated with DMSO (lane 1–3) or TSA (lane 4–6) and recombinant human histones loaded as reference (lane 7–9). b Acetylation of K562 histones was validated by western blotting using anti-H3K27ac primary antibody (ab177178, Abcam) and anti-H3 primary antibody (ab1791, Abcam) as reference. c GST pull-down assay performed with GST-fused c-Myb DBD (NR123) and 6 µg native K562 histone octamers. The western blot was analysed by using anti-H3 and anti-H3K27ac primary antibodies. About 10% (0.6 µg) of the K562 histones was loaded as reference