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Table 2 PCR primer sequences for bisulfite sequencing analysis

From: Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice

  Names Sequences
5′ primers lambda-MA-5S1 5′-attagtaagaagatagtagtgatg-3′
lambda-MA-5S4 5′-ttaagttttgtgtgttatttatta-3′
lambda-MA-5S5 5′-gttaaaaagaagaagtaagtattt-3′
lambda-MA-5S6 5′-gtgaaagtattgattattatgtta-3′
lambda-MA-5S7 5′-gaggtttatttgtatttatttttgtt-3′
lambda-MA-5S8 5′-tattttttagtagtattgtaagaggt-3′
3′ primers lambda-MA-3A2 5′-ataccttatttttttctactacaa-3′
lambda-MA-3A3 5′-ctaaactccaacatataataaccc-3′
lambda-MA-3A7 5′-aaccaaaattatctttttctatct-3′
lambda-MA-3A8 5′-acaacattcttaaatccaatatta-3′
BGLB-MA-3A2 5′-ttctaaccccacaaaaatttattc-3′
BGLB-MA-3A6 5′-ccaaaccccctctattttatatca-3′