Consistent inverse correlation between DNA methylation of the first intron and gene expression across tissues and species

Table 1 Effects on gene expression of the methylation status and the relative distance of the CpGs inside the four transcription factor binding motifs enriched in the first introns

Tissue	Factors	SS	d.f.	F value	p value
Muscle	Relative distance	5.18	1	1.872	0.173
	Methylation status	10.76	1	3.885	0.051
	Interaction of relative distance with methylation status	17.35	1	6.264	0.013
	Residuals	387.65	140
Testis	Relative distance	7.31	1	1.855	0.176
	Methylation status	121.86	1	30.900	0.000
	Interaction of relative distance with methylation status	22.8	1	5.781	0.018
	Residuals	540.28	137

The effects were tested using analysis of covariance, and the statistically significant ones are shown in italics
d.f. degrees of freedom, SS sums of squares

ISSN: 1756-8935