Fig. 6

Differentially expressed genes with differentially methylated regions (tDMRs) between tissues. tDMRs overlap with the gene body and/or  4 kb upstream from the transcription start site or downstream of the 3’ UTR (a, n = 1044), the promoter (b, n = 47), the first exon (c, n = 75) or the first intron (d, n = 187). Positive (circles) and negative (boxes) correlation is shown for up-regulated genes in muscle (red) and up-regulated genes in testis (blue). Hyper-methylated tDMRs and up-regulated DEGs in testis (blue circles), hypo-methylated tDMRs and up-regulated DEGs in testis (blue squares), hypo-methylated tDMRs and up-regulated DEGs in muscle (red squares) and hyper-methylated tDMRs and up-regulated DEGs in muscle (red circles). Differentially expressed were considered the genes with log₂ fold change > |1.5| and false discovery rate < 0.05. tDMRs were defined as regions showing more than 15% methylation difference between tissues and q value < 0.001, with a minimum number of 5 CpGs and 3 differentially methylated cytosines (DMCs), where a DMC showed more than 15% methylation difference between tissues. Transcription factor binding motifs present in the tDMRs of gene bodies and/or ± 4 kb that are common between positive and negative correlation of DNA methylation with gene expression or that are correlation specific (negative or positive)