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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Episomal HBV persistence within transcribed host nuclear chromatin compartments involves HBx

Fig. 1

Correlative analyses of HBx-hepatocyte genome association, transcriptomics, Pol2 and several chromatin structure markers on chromosomes 1, 3, 15 and Y in MMH-D3 cells. a We performed correlation analyses of NLS-HBx-RFP with respect to Pol2 and several histone modifications. To assess the resolution of putative correlation, we applied different bin sizes for analyses. The graphs illustrate exemplarily for chromosome 15, how R2 increases for Pol2, H3K36me3, H3K36ac and H3K4me3, when larger resolution windows of 50 or 250 kb were selected. b This sub-figure comprehensively outlines massive parallel sequencing derived whole-genome data for chromosomes 1, 3, 15 and Y (from top to bottom). For each chromosome, the following data are presented (in the order from top to bottom): chromosomal structure, transcriptome, GC-content (both obtained from the UCSC genome browser), ChIP-seq derived enrichment of Pol2, NLS-HBx-RFP, H3K36ac, H3K36me3, H3K4me3, H3K4me1, H3K27ac, H3K27me3, H3K9ac and H3K9me3. Below, the position of RefSeq genes is indicated (obtained from the UCSC genome browser). The degree of enrichment is color-coded as indicated in the legend on the bottom of the figure. On four different chromosomes, this figure demonstrates a correlation using a 50 kb bin size for analyses of HBx mapping and gene transcription, Pol2 mapping as well as enrichment of PTMs that have been associated with active gene transcription. In contrast, repressive PTMs do not exhibit a correlative mapping pattern with HBx enrichment

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