Fig. 5

The relationship between the expression of BmDnmt1, BmDeaf1 and BmCHSA-2b in the wing disk. A qRT-PCR analyses of BmCHSA-2b (black), BmDnmt1 (blue) and BmDeaf1 (red) in the wing disk from 1-day-old fifth instar larvae (5LD1) to 6-day-old pupae (PD6). B Western blot analyses of BmDnmt1 (left) and BmDeaf1 (right) protein levels in the wing disk at the stages of 5LD3, 5LD5, W0 (the initiation of the wandering stage), PD2 and PD4. The upper panels are the quantitative counts of the bands of target proteins by Western blots in the bottom panels. BmTublin was used to show equal loading of proteins. For the T test: p < 0.05 (*) or p < 0.01(**). C Immunohistochemistry analyses of BmDnmt1 and BmDeaf1 protein levels in the wing disk of fifth instar larvae, wandering larvae and pupa: (a-l) BmDnmt1 protein level at 5LD3 (a-d), W (e–h), PD4 (i-l); (m-x) BmDeaf1 protein level at 5LD3 (m-p), W (q-t), PD4 (u-x). 5LDn: n-day-old fifth instar larvae. W wandering stage when the larvae stop feeding, PDn n-day-old pupae, HE hematopoiesis, WB wing base, WC wing cell. Scale bar: 500 μm for a–b and m–n; 1 mm for e–f and q–r; 4 mm for i–j and u–v; 100 μm for c–d, g–h, k–l, o–p, s–t and w–x. Blue: DAPI, red in a-l: BmDnmt1 protein, red in m–x: BmDeaf1 protein