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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori

Fig. 3

The regulatory effect of BmDeaf1 on BmCHSA-2b promoter activity. a mRNA level of BmDnmt1 in larvae (Day 3 of fifth instar larval stage), pupae (Day 3 of pupal stage) and the Bm12 cell line. b Change of BmDeaf1 mRNA level after dsBmDeaf1 transfected into the Bm12 cells. c Changes in the luciferase activities under the control of the wild-type or mutant CpGI2 promoter in the Bm12 cells co-transfected with dsBmDeaf1 or dsgfp (as control) and the CpGI2 promoter luciferase vector (pWT-CpGI2-Luc) or the CpGI2-mutated promoter luciferase vector (pMut-CpGI2-Luc). d Changes in the luciferase activity under the control of the wild-type CpGI2 promoter in the Bm12 cells co-transfected with dsBmDeaf1, dsBmDnmt1 and the CpGI2 promoter luciferase vector (pWT-CpGI2-Luc). e Changes in the luciferase activity under the control of the CpGI2 promoter in the Bm12 cells co-transfected BmDeaf1-EGFP vector, BmDnmt1-EGFP vector and pWT-CpGI2-Luc. f Changes in the luciferase activity under the control of the wild-type or mutated CpGI2 promoter in the Bm12 cells co-transfected with pWT-CpGI2-Luc or pMut-CpGI2-Luc and EGFP vector (as control) or BmDnmt1-EGFP vector or BmDnmt1-EGFP vector followed by BmDeaf1-EGFP vector 24 h later. For the t test: p < 0.01(**). The sequences of the wild-type and mutant CpGI 2 are shown in Additional file 10: Table S1

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