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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori

Fig. 1

Identification of the methylation sequences in the promoter region of the BmCHSA-2b gene. a BmCHSA gene structure showing the positions of two promoters (P1 and P2) and the three CpG islands in the 2 kb of promoter P2 and 5′ untranslated region of BmCHSA-2b. The blue boxes show the exons and the red boxes show the 5′-UTR of BmCHSA-2a and BmCHSA-2b. The tubular lines show the region of the introns and the promoters of BmCHSA-2a and BmCHSA-2b. The light blue shapes show the regions of CpGI1, 2 and 3. b The methylation rate analysis of CpGI1, 2 and 3 in the BmCHSA-2b promoter in the 3-day-old pupal wings (WD, red line) and epidermis (EP, blue line) by bisulfite sequencing PCR (BSP). The bolded blue lines show the methylation positions of the CpGI1, 2 and 3. c The enlarged and detailed figure of the squared region in b, showing the sequence of the detailed nucleotides and the methylation positions in the CpGI2. All data included three biological replicates, each with nine individual repeats. For the t test: p < 0.05 (*) or p < 0.01(**)

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