Fig. 4

Quantitative analysis of Commd1 expression and Zrsr1-DMR methylation in ΔPA blastocysts and the ΔPA adult brain. a Allelic expression of Commd1 was analyzed in Commd1^{+(B6)/+(BALB)} (WT), Commd1^{PA(B6)/+(BALB)} (PA), and Commd1^{ΔPA(B6)/+(BALB)} (ΔPA) blastocysts in triplicate (shown with n) by pyrosequencing. Alleles were discriminated between using an SNP between B6 (Mat) and BALB/c (Pat) located in exon 2 (rs26846230; C in B6, T in BALB/c). The allelic expression ratios are presented relative to paternal expression in each blastocyst sample. b Methylation at Zrsr1-DMR was analyzed in the blastocysts used in a. The 343-bp region in the DMR containing 25 CpG sites (B6; maternal allele) or 24 CpG sites (BALB/c; paternal allele) was analyzed via bisulfite sequencing. The alleles were discriminated between using an SNP (rs26846192; C in B6, A in BALB/c) located in a CpG site in the B6 sequence. Closed and open circles depict methylated and unmethylated CpGs, respectively. c Methylation at Zrsr1-DMR was analyzed in the brains of ΔPA and WT adult F1 mice as described in b. d Allelic expression of Commd1 in the adult F1 brain was quantitatively analyzed in triplicate (shown with n) by pyrosequencing and is presented as in a. e Total Commd1 expression in an adult F1 brain of each genotype was analyzed in triplicate (shown with n) via TaqMan RT-PCR. Total expression is presented relative to the WT expression level. Asterisks (*) indicate statistical significance (P < 0.01) according to the two-sided Student’s t test