Fig. 3
From: Growing oocyte-specific transcription-dependent de novo DNA methylation at the imprinted Zrsr1-DMR
Analysis of Commd1 and Zrsr1 expression and Zrsr1-DMR methylation in adult mice. a Allelic expression of Commd1 was analyzed as in Fig. 2a in the brain (Br) and liver (Lv) of Commd1PA(B6)/+(PWK) (PA) and Commd1+(B6)/+(PWK) (WT) adult F1 mice. MW: molecular weight marker. B6 and PWK are the maternal and paternal alleles in the F1 mice, respectively. b Methylation at Zrsr1-DMR was analyzed as in Fig. 2b in the brain of adult F1 mice. Shown is the SNP used to discriminate the parental alleles. c Allelic expression of Zrsr1 was analyzed in the F1 adult brain by direct RT-PCR sequencing of the cDNA, which contains two SNP sites between B6 and PWK alleles. d Total Zrsr1 expression was analyzed in triplicate (shown with n) in the adult F1 brain and liver via TaqMan RT-PCR. Total expression is presented relative to the WT expression level in each tissue. The differences in levels of expression between WT and ΔPA F1 mice were statistically significant in the brain and liver (two-sided Student’s t test, *P < 0.01)