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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5

Fig. 3

Hsp90 and Mas5 are required for the assembly of heterochromatin at the pericentromere. a, b Strand-specific RT-qPCR for the pericentromeric transcripts from the ade6 gene and dg repeats (a), and for the subtelomeric transcripts from the tlh genes (b). For the dg repeats and tlh genes, transcripts matching forward (Fw) and reverse (Rv) strands were analyzed. Values are normalized to that of the sense strand of ribosomal 28S RNA. c ChIP-qPCR using an antibody against H3K9me2 for the pericentromeric ade6 gene, dg repeats, and tlh genes. Color keys under the graphs in ac correspond to the key shown in a. The euchromatic gene act1 was used as an internal control locus. d ChIP-qPCR of Myc-Ago1 using an antibody against Myc for the pericentromeric ade6 gene, dg repeats, and tlh genes. For all panels, values are presented as means + SD (n = 3)

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