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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation

Fig. 6

CBP30 displaces CREBBP and EP300 from chromatin at GATA1/MYC binding sites in K562 cells. a Heat map of the density of EP300, H3K27ac, GATA1 and MYC signals at 10 Kb centered of all EP300-occupied intervals (n = 76,018) and ranked according to EP300 signal. b Overlap of genes occupied by GATA1, MYC and top levels of EP300. c ChIP-seq profiles of H3K27ac, EP300, GATA1 and MYC at given genomic regions. Red lines show the location of super-enhancers. Gray lines show the genomic intervals with top EP300 occupancy. Regions A, B and C were used for ChIP-qPCR. Magnifications of regions B and C are also shown. d ChIP-qPCR showing enrichment of CREBBP and EP300 in K562 cells treated with vehicle (DMSO) and 5 µM CBP30 for one hour in regions A, B and C. Levels were normalized to the input and plotted relative to the IgGs levels in the vehicle-treated condition. The position of the amplicons relative to the transcription start site of each gene is indicated. A region downstream of the gene HMGA2 was used as a negative control. e ChIP-qPCR showing enrichment of H3K27ac in K562 cells treated with vehicle (DMSO) and 5 µM CBP30 for one hour in regions A, B and C. Levels were normalized to the input and plotted relative to the IgGs levels in the vehicle-treated condition. The position of the amplicons relative to the transcription start site of each gene is indicated. A region downstream of the gene HMGA2 was used as a negative control. *p value < 0.05, **p value < 0.005 and ***p value < 0.0005 determined by t test

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