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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: DamID profiling of dynamic Polycomb-binding sites in Drosophila imaginal disc development and tumorigenesis

Fig. 5

Polycomb-binding transitions fail in scrib1 imaginal discs development. a Pearson’s correlations between DamID-seq Pc profiles obtained from WIDs in early larval stages (Early), late larval stages (Late) and in scrib1. bb′ Percentage of GATC fragments that classify as loss, gain and no change in Pc-binding states in (b) late (WT Late) if compared to early (WT Early) WIDs and (b′) in scrib1 WIDs if compared to early (WT Early) WIDs. Loss, gain and no-change transitions were determined by transitions between ‘enriched’, ‘intermediate’ and ‘depleted’ Pc-binding states classified by a three-state HMM analysis. Note that the no-change category contains GATC fragments that were classified as ‘enriched’, ‘intermediate’ and ‘depleted’ for Pc-binding and thus includes Pc target and non-target genes. c Relationship of Pc-targeted GATC fragments that classify as loss, gain and no change in Pc-binding in ‘scrib1 if compared to late (WT Late) WIDs’ versus ‘late (WT Late) if compared to early (WT Early) WIDs’ (gain—orange, loss—light blue, no change—grey). The dotted frame highlights sites that lost Pc-binding in scrib1 if compared to WT Late samples but should have gained Pc-binding during normal wing disc development. d Regulatory elements identified by i-cisTarget that represent enrichment for chromatin-binding factors and transcription factors at GATC fragments conservatively classified as ‘enriched’ for Pc-binding in early WID samples and on GATC fragments classified as a gain (Late gain) or loss (Late loss) of Pc-binding by transitioning in and out of the ‘enriched’ state in late developmental stages if compared to an earlier stage. Normalized enrichment scores (NES) are visualized as coloured scale

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