Fig. 5

Polycomb-binding transitions fail in scrib¹ imaginal discs development. a Pearson’s correlations between DamID-seq Pc profiles obtained from WIDs in early larval stages (Early), late larval stages (Late) and in scrib¹. b–b′ Percentage of GATC fragments that classify as loss, gain and no change in Pc-binding states in (b) late (WT Late) if compared to early (WT Early) WIDs and (b′) in scrib¹ WIDs if compared to early (WT Early) WIDs. Loss, gain and no-change transitions were determined by transitions between ‘enriched’, ‘intermediate’ and ‘depleted’ Pc-binding states classified by a three-state HMM analysis. Note that the no-change category contains GATC fragments that were classified as ‘enriched’, ‘intermediate’ and ‘depleted’ for Pc-binding and thus includes Pc target and non-target genes. c Relationship of Pc-targeted GATC fragments that classify as loss, gain and no change in Pc-binding in ‘scrib¹ if compared to late (WT Late) WIDs’ versus ‘late (WT Late) if compared to early (WT Early) WIDs’ (gain—orange, loss—light blue, no change—grey). The dotted frame highlights sites that lost Pc-binding in scrib¹ if compared to WT Late samples but should have gained Pc-binding during normal wing disc development. d Regulatory elements identified by i-cisTarget that represent enrichment for chromatin-binding factors and transcription factors at GATC fragments conservatively classified as ‘enriched’ for Pc-binding in early WID samples and on GATC fragments classified as a gain (Late gain) or loss (Late loss) of Pc-binding by transitioning in and out of the ‘enriched’ state in late developmental stages if compared to an earlier stage. Normalized enrichment scores (NES) are visualized as coloured scale