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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: DamID profiling of dynamic Polycomb-binding sites in Drosophila imaginal disc development and tumorigenesis

Fig. 1

Establishing cell lineage-specific DamID in wing imaginal discs. a Schematic representation of the FLP-inducible Dam and Dam-Pc constructs used in this study. b Characteristic DNA smear formed by DamID methylation-dependent PCR products on agarose gel. Lanes 1–2: wing imaginal disc (WID) samples, where FLP expression has not been induced. Lanes 3–4: WID samples from genotypes ubiquitously expressing FLP after induction by a heat shock (hsflp). me-PCR-NC refers to negative PCR controls lacking DNA template. c, d WIDs stained for expression of the Myc-tag if Dam (c) and Dam-Pc (d) were induced by ptcGAL4-driven expression of UAS-FLP(EXEL). Expression of the Myc-tagged fusion proteins was boosted by a heat shock (see Experimental procedures). A and P refer to anterior and posterior compartments, respectively. ee″ Wild-type WID stained with DAPI (e), and for pH3 (e′) and Dcp-1 (e″). Maximum projections of a confocal stack are shown in E’ and E’’ to reveal all signals. ff″ WID from ptc > FLP;Dam-Pc expressing larvae stained with DAPI (F) and for pH3 (f′) and Dcp-1 (f″). Maximum projections of a confocal stack are shown in f′ and f″ to reveal all signals. gg″ WIDs from indicated genotypes stained for patched (Ptc). A and P refer to anterior and posterior compartments, respectively. h–h″ WIDs from indicated genotypes stained for wingless (Wg). D and V refer to dorsal and ventral compartments, respectively. ii″ Adult wings from indicated genotypes 24 h after eclosion. All scale bars: 100 µm

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