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Table 8 Comparison of coverage output of FFPET on the HiSeq X Ten using two library preparation kits

From: Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten

Library preparation method Library preparation kit name Input amount (ng) DNA samples FFPET Sequencing platform Fragment size (stdev) Duplicate read (%) Coverage/lane CpG island/shorea
Post-BS Accel-NGS Methyl-Seq 100 1. Prostate normal(1601) HiSeq X Ten 158 bp (51 bp) 13 13.05× 0.6:1.0
  2. Prostate cancer (1601) 177 bp (53 bp) 18 13.97× 0.4:0.9
TruMethyl WG v1.9 200 1. Prostate normal (1601) 161 bp (81 bp) 23 11.16× 1.3:1.3
  2. Prostate cancer (1601) 174 bp (91 bp) 27 10.84× 0.8:1.1
  1. aRatio of coverage represented in the CpG islands and shores of the genome