Table 8 Comparison of coverage output of FFPET on the HiSeq X Ten using two library preparation kits
Library preparation method | Library preparation kit name | Input amount (ng) | DNA samples FFPET | Sequencing platform | Fragment size (stdev) | Duplicate read (%) | Coverage/lane | CpG island/shorea |
|---|---|---|---|---|---|---|---|---|
Post-BS | Accel-NGS Methyl-Seq | 100 | 1. Prostate normal(1601) | HiSeq X Ten | 158 bp (51 bp) | 13 | 13.05× | 0.6:1.0 |
2. Prostate cancer (1601) | 177 bp (53 bp) | 18 | 13.97× | 0.4:0.9 | ||||
TruMethyl WG v1.9 | 200 | 1. Prostate normal (1601) | 161 bp (81 bp) | 23 | 11.16× | 1.3:1.3 | ||
2. Prostate cancer (1601) | 174 bp (91 bp) | 27 | 10.84× | 0.8:1.1 |