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Table 5 Comparison of coverage from bisulphite and genomic library when sequenced in a 50:50 ratio on a single lane of HiSeq X Ten

From: Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten

Library preparation method Library preparation kit name Input amount (ng) WGBS library sample WGBS library loading concentration WGS library samplea % of spike-in library WGBS coverage/lane WGS coverage/lane
Post-BS TruMethyl WG v1.9 200 1a. Prostate cancer DNA (5287) 250 pM 1a. Prostate cancer DNA (5287) 50% 13.48× 13.48×
1b. Prostate cancer DNA (5287) 1b. Prostate cancer DNA (5287) 13.16× 13.97×
2a. Prostate cancer DNA (5060) 2a. Prostate cancer DNA (5060) 13.20× 15.38×
2b. Prostate cancer DNA (5060) 2b. Prostate cancer DNA (5060) 12.70× 15.70×
3a. Prostate cancer DNA (13179) 3 a. Prostate cancer DNA (13179) 10.70× 16.12×
3b. Prostate cancer DNA (13179) 3b. Prostate cancer DNA (13179) 9.80× 16.83×
4a. Prostate cancer DNA (10738) 4a. Prostate cancer DNA (10738) 11.80× 16.50×
4b. Prostate cancer DNA (10738) 4b. Prostate cancer DNA (10738) 11.20× 16.45×
  1. a50% of corresponding genomic library used as spike-in