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Table 5 Comparison of coverage from bisulphite and genomic library when sequenced in a 50:50 ratio on a single lane of HiSeq X Ten

From: Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten

Library preparation method

Library preparation kit name

Input amount (ng)

WGBS library sample

WGBS library loading concentration

WGS library samplea

% of spike-in library

WGBS coverage/lane

WGS coverage/lane

Post-BS

TruMethyl WG v1.9

200

1a. Prostate cancer DNA (5287)

250 pM

1a. Prostate cancer DNA (5287)

50%

13.48×

13.48×

1b. Prostate cancer DNA (5287)

1b. Prostate cancer DNA (5287)

13.16×

13.97×

2a. Prostate cancer DNA (5060)

2a. Prostate cancer DNA (5060)

13.20×

15.38×

2b. Prostate cancer DNA (5060)

2b. Prostate cancer DNA (5060)

12.70×

15.70×

3a. Prostate cancer DNA (13179)

3 a. Prostate cancer DNA (13179)

10.70×

16.12×

3b. Prostate cancer DNA (13179)

3b. Prostate cancer DNA (13179)

9.80×

16.83×

4a. Prostate cancer DNA (10738)

4a. Prostate cancer DNA (10738)

11.80×

16.50×

4b. Prostate cancer DNA (10738)

4b. Prostate cancer DNA (10738)

11.20×

16.45×

  1. a50% of corresponding genomic library used as spike-in