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Table 3 Comparison of coverage from bisulphite and genomic library when 25% of genomic library is spiked

From: Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten

Library preparation method Library preparation kit name Input amount (ng) WGBS library samples Sequencing platform WGBS library loading concentration WGS library samplea % of spike-in library WGBS coverage/lane WGS coverage/lane
Post-BS TruMethyl WG v1.9 200 1. Cell line (LNCaP) HiSeq X Ten 250 pM Cell line (LNCaP) 25% 15.57× 7.40×
2. Cell line (LNCaP) 14.10× 7.02×
3. Cell line (B80-T17-P12) 16.50× 9.50×
4. Blood sample 3 14.80× 5.47×
5. Blood sample 4 14.40× 12.47×
  1. aGenomic library used as spike-in