Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten

Table 3 Comparison of coverage from bisulphite and genomic library when 25% of genomic library is spiked

Library preparation method	Library preparation kit name	Input amount (ng)	WGBS library samples	Sequencing platform	WGBS library loading concentration	WGS library sample^a	% of spike-in library	WGBS coverage/lane	WGS coverage/lane
Post-BS	TruMethyl WG v1.9	200	1. Cell line (LNCaP)	HiSeq X Ten	250 pM	Cell line (LNCaP)	25%	15.57×	7.40×
			2. Cell line (LNCaP)					14.10×	7.02×
			3. Cell line (B80-T17-P12)					16.50×	9.50×
			4. Blood sample 3					14.80×	5.47×
			5. Blood sample 4					14.40×	12.47×

ISSN: 1756-8935