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Table 2 Comparison of coverage obtained with different bisulphite library and PhiX loading concentrations

From: Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X Ten

Library preparation method Library preparation kit name Input amount (ng) DNA samples Sequencing platform Bisulphite library loading concentration PhiX loading concentration % of spike-in Coverage/lane
Post-BS TruMethyl WG v1.9 200 Cell line (LNCaP) HiSeq X Ten 300 pM 250 pM 25% 8.70×
Cell line (LNCaP) 250 pM 250 pM 14.50×
Cell line (LNCaP) 300 pM 15.19×
1. Blood sample (2) 250 pM 15.46×
2. Blood sample (3) 250 pM 13.87×
3. Blood sample (4) 250 pM 12.15×
4. Blood sample (6) 300 pM 20.24×
5. Blood sample (23) 300 pM 16.59×
6. Blood sample (24) 300 pM 19.67×