Fig. 2From: Guidelines for whole genome bisulphite sequencing of intact and FFPET DNA on the Illumina HiSeq X TenOptimisation of spike-in library and its loading concentration. a Bar plot showing the difference in coverage obtained from sequencing two different loading concentrations of the PhiX spike-in library for six WGBS libraries obtained from blood DNA samples. b Bar plot showing the coverage obtained from both the bisulphite library and genomic library when 25% of genomic library is spiked in instead of PhiX, for five different bisulphite libraries prepared by the TruMethyl WG method. c Box plot showing the coverage distribution of the same set of bisulphite libraries when spiked with either 25% of 250 pM genomic library or 25% of 300 pM of PhiX libraryBack to article page