Fig. 4

Targeted bisulfite pyrosequencing-based DNA methylation differences of Fyn in control, F1-, F2-, and F3-GDM male mice. a Methylation differences in D13.5 PGCs from control and GDM male fetuses (n = 5 pregnant mice per group). b Methylation differences in D18.5 control, F1-, and F2-GDM testes (n = 8 mice per group). c Methylation differences in sperm from control, F1/C-GDM, and F2/C-GDM mice at 8 weeks (n = 5 mice per group). d Methylation differences in D7.5 fetuses from control, F2-, and F3-GDM mice (n = 5 mice per group). e Methylation differences in adipose tissues from control, F2/H-GDM, and F2/N-GDM male mice at 8 weeks (n = 5 mice per group). f Methylation differences in liver tissues from control, F2/H-GDM, and F2/N-GDM male mice at 8 weeks (n = 5 mice per group). g Methylation differences in muscle tissues from control, F2/H-GDM, and F2/N-GDM male mice at 8 weeks (n = 5 mice per group). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding control