Fig. 3

Strand symmetry of methylation values MA-plots. MA-plots of the log average of the methylation levels (A) on the x-axis and log ratio of the methylation levels (M) on the y-axis, between complementary CpG positions. Median absolute deviation (MAD) values are used to evaluate the agreement in methylation levels. The bimodal nature of methylation patterns (mostly unmethylated or methylated) is reflected in the high density at both ends of the x-axis. The artificially discordant sites introduced during ERRBS library preparation are identified as increased density off the center line at the low methylation values (at A < 0 range). a ERRBS_A, b ERRBS_B, c SSMethylSeq_A, d SSMethylSeq_B, e CpGiant_A, f CpGiant_B, g TruseqEpic, and h WGBS-PBAT