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Table 1 Fertility tests

From: ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization

Females

Temperature (°C)

Males

Number of eggs

Hatching rate (%)

MTD-Gal4 > UAS-shRNA asf1

25

w 1118

1715

0

nos-Gal4 > UAS-shRNA asf1

25

w 1118

987

0

MTD-Gal4 > UAS-shRNA asf1

25

g-cid-EGFP::cid

276

0

MTD-Gal4 > UAS-shRNA Hira

25

g-cid-EGFP::cid

354

0

MTD-Gal4 > UAS-shRNA yem

25

g-cid-EGFP::cid

489

0

UAS-asf1::V5(62E), UAS-shRNA asf1/TM6

25

w 1118

295

91.07

MTD-Gal4 > UAS-asf1::V5(62E), UAS-shRNA asf1

25

w 1118

674

92.41

MTD-Gal4 > UAS-asf1::V5(62E), UAS-shRNA asf1

29

w 1118

462

80.97

g-asf1::V5(62E), asf12/asf11

25

g-asf1::V5(62E), asf12/asf11

172

66.2

g-asf1::V5(62E), asf12/asf11

25

w 1118

40

67.2

g-asf1::V5(62E), UAS-shRNA asf1/TM6B

25

w 1118

349

78.7

g-asf1::V5(62E), UAS-shRNA asf1

25

w 1118

530

74.9

g-asf1::V5(62E), MTD-Gal4 > UAS-shRNA asf1

25

w 1118

1891

5.3

g-asf1::V5(62E), MTD-Gal4 > UAS-shRNA asf1

29

w 1118

1314

0