Fig. 6
The HIRA B domain is dispensable for sperm chromatin remodeling. a Representation of HIRA::Flag proteins expressed from transgenes used in c-e. The position of WD40 repeats and B domain are shown. The ability of each corresponding transgene to rescue the fertility of homozygous HiraHR1 females (embryo hatching rate) is indicated. b Alignment of human (Hs) or Drosophila (Dm) HIRA B domains. Conserved residues are highlighted in red. The deleted residues in HIRA[ΔB]-Flag are indicated. c Representative images (merged DNA and H4act stainings) of pronuclear apposition (upper panels) and first zygotic anaphase (lower panels) from the indicated females. Scale bar: 10 μm. d Quantification of pronuclear size at apposition for the indicated genotypes are represented as a scatter plot. Horizontal lines represent means. The number of pronuclei analyzed is shown under the graph. In HiraHR1, Hira∆B::Flag flies, the size of male pronucleus relative to the female counterpart is significantly smaller than in control flies (Unpaired t test: p < 0.0001 Hira∆B::Flag versus wild type and p = 0.0004 Hira∆B::Flag versus Hira::Flag). e Co-immunoprecipitation experiments performed with anti-FLAG (HIRA) and anti-V5 (ASF1) on ovarian extracts from females carrying the indicated transgenes and mutant alleles. Deletion of the HIRA B domain does not abolish the ASF1-HIRA interaction in ovaries