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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization

Fig. 3

Depletion of maternal ASF1 does not prevent embryo development. a In control early syncytial embryos (left panels), ASF1 accumulates in nuclei at each synchronous S phase (n = 24). Note that ASF1 is not detected in the rosette of condensed chromosomes that form the polar body (asterisk). In embryos from asf1 KD females (right panels), nuclear amplification is observed despite the apparent absence of ASF1 (n = 28). Scale bar: 10 μm. b A syncytial blastoderm embryo showing the accumulation of ASF1::V5 in replicating nuclei. ASF1::V5 is expressed from a g-asf1::V5 transgene and is detected with an anti-V5 antibody. c Quantification of embryonic phenotypes corresponding to the indicated genotypes. Embryos were collected every 2 h and then allowed to develop for 2 h at 25 °C before fixation and developmental stage determination. A minimum of 150 embryos were scored for each genotype. Corresponding raw data are shown in Additional file 3: Table S1. d asf1 KD rescue with a UAS-asf1::V5 transgene. Top panel: A representative stage 13 embryo from a asf1 KD female expressing the rescue transgene UAS-asf1::V5 (n = 62). Lower panel: A representative late asf1 KD embryo at approximately the same stage showing aberrant segmentation (n = 57). Color panels in a and d show merged stainings. Scale bar: 50 μm

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