Fig. 1
asf1 knock-down and rescue experiments. a Schemes of the D. melanogaster asf1 gene and rescue transgenes used in this study. The respective target sites of the GL00171 and HMC03937 shRNAs are indicated. b Left: a control P[TRiP.HMC03937]/+ adult showing normal wings. Right: induction of the HMC03937 shRNA in the wing imaginal disk with vg-Gal4 results in severe wing atrophy in all tested flies (n = 224). c Western blot analysis of endogenous or V5-tagged ASF1 extracted from ovaries or embryos of indicated genotypes. The anti alpha-Tubulin immunostaining was used as a loading control. Note that the UAS-V5-asf1 transgene does not contain the 21-bp target of the asf1 GL00171 shRNA. The anti-ASF1 antibody recognizes both wild-type and tagged versions of ASF1. Arrows indicate specific bands