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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Deletion of HP1γ in cardiac myocytes affects H4K20me3 levels but does not impact cardiac growth

Fig. 6

Effect of HP1γ KO on TAC-induced hypertrophy. a TAC-induced heart growth. TAC or Sham surgeries were performed at 10–12 weeks and hearts were harvested 1 week after operations. Heart weight were measured and normalized by BW. Each data point indicates a value from individual animals. Data from male are shown in blue and females in red. *p < 0.05. b Cross-sectional area after TAC. Paraffin heart sections were stained with WGA. Scale bar indicates 50 μm. c Heart tissues were stained with cell cycle marker Ki67 (white), Hoechst (red) and WGA (green). Individual channel images are available in Additional file 12: Fig. S8. Scale bar indicates 100 μm. d, e Expression of cardiac and cell cycle genes. Total RNA was extracted from isolated CMs 1 week after TAC and quantitative RT-PCR was performed using gene specific primers. Gene expression was normalized against expression of internal control S26 and shown as relative expression value against Sham operated fl/fl. S26 gene expression did not change by neither operation nor genotype

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